Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease
Identifieur interne : 001453 ( Main/Exploration ); précédent : 001452; suivant : 001454Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease
Auteurs : Susan Pearce-Kelling [États-Unis] ; William J. Mitchell [États-Unis] ; Brian A. Summers [États-Unis] ; Max J. G. Appel [États-Unis]Source :
- Microbial Pathogenesis [ 0882-4010 ] ; 1990.
English descriptors
- Teeft :
- Appel, Astrocyte, Astrocyte infection, Astrocyte population, Astrocytic infection, Basal medium, Brain cell cultures, Brain cell passaged, Brain cells, Canine, Canine brain cell cultures, Canine distemper virus, Cell types, Comparative studies, Cornell university, Cultured astrocytes relationship, Days post inoculation, Demyelinating, Distemper, Distemper virus, Encephalitis, Encephalomyelitis, Fresh brain cell cultures, Gfap staining, Gray matter, Harleco corp, Infection, Infectious virus, Inoculation, Lung macrophages, Multinucleated syncytia, Oligodendrocyte, Rapid infection, Rapid rise, Same field, Shorr stain, Subacute, Supernatant, Supernatant fluid, Supernatant fluids, Time point, Vaccinal strain, Veterinary medicine, Viral, Viral antigen, Viral inclusions, Viral strain, Viral strains, Virulent, Virulent strains, Virus strain, Vivo persistence, White matter, White matter disease, York state college.
Abstract
Abstract: Canine distemper virus (CDV) causes an encephalomyelitis in dogs which varies with the viral strain. The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes in vitro, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains in vivo may be related to the course of astrocyte infection observed in vitro.
Url:
DOI: 10.1016/0882-4010(90)90009-F
Affiliations:
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Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853</wicri:regionArea><orgName type="university">Université Cornell</orgName><placeName><settlement type="city">Ithaca (New York)</settlement><region type="state">État de New York</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Microbial Pathogenesis</title><title level="j" type="abbrev">YMPAT</title><idno type="ISSN">0882-4010</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">8</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="71">71</biblScope><biblScope unit="page" to="82">82</biblScope></imprint><idno type="ISSN">0882-4010</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0882-4010</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Appel</term><term>Astrocyte</term><term>Astrocyte infection</term><term>Astrocyte population</term><term>Astrocytic infection</term><term>Basal medium</term><term>Brain cell cultures</term><term>Brain cell passaged</term><term>Brain cells</term><term>Canine</term><term>Canine brain cell cultures</term><term>Canine distemper virus</term><term>Cell types</term><term>Comparative studies</term><term>Cornell university</term><term>Cultured astrocytes relationship</term><term>Days post inoculation</term><term>Demyelinating</term><term>Distemper</term><term>Distemper virus</term><term>Encephalitis</term><term>Encephalomyelitis</term><term>Fresh brain cell cultures</term><term>Gfap staining</term><term>Gray matter</term><term>Harleco corp</term><term>Infection</term><term>Infectious virus</term><term>Inoculation</term><term>Lung macrophages</term><term>Multinucleated syncytia</term><term>Oligodendrocyte</term><term>Rapid infection</term><term>Rapid rise</term><term>Same field</term><term>Shorr stain</term><term>Subacute</term><term>Supernatant</term><term>Supernatant fluid</term><term>Supernatant fluids</term><term>Time point</term><term>Vaccinal strain</term><term>Veterinary medicine</term><term>Viral</term><term>Viral antigen</term><term>Viral inclusions</term><term>Viral strain</term><term>Viral strains</term><term>Virulent</term><term>Virulent strains</term><term>Virus strain</term><term>Vivo persistence</term><term>White matter</term><term>White matter disease</term><term>York state college</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Canine distemper virus (CDV) causes an encephalomyelitis in dogs which varies with the viral strain. The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes in vitro, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains in vivo may be related to the course of astrocyte infection observed in vitro.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Pearce Kelling, Susan" sort="Pearce Kelling, Susan" uniqKey="Pearce Kelling S" first="Susan" last="Pearce-Kelling">Susan Pearce-Kelling</name></region><name sortKey="Appel, Max J G" sort="Appel, Max J G" uniqKey="Appel M" first="Max J. G." last="Appel">Max J. G. Appel</name><name sortKey="Mitchell, William J" sort="Mitchell, William J" uniqKey="Mitchell W" first="William J." last="Mitchell">William J. Mitchell</name><name sortKey="Summers, Brian A" sort="Summers, Brian A" uniqKey="Summers B" first="Brian A." last="Summers">Brian A. Summers</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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